2014 Fiscal Year Final Research Report
Establishment of tooth germ regeneration using neural crest derived cells
| Project/Area Number |
25861896
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Showa University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 神経堤細胞 / 毛包 / 再生医学 / 硬組織再生 / 歯胚再生 |
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| Outline of Final Research Achievements |
Neural crest(NC) cells are maintained in an undifferentiated state as NC-derived cells (NCDCs) throughout the life of the animal. We utilized P0-Cre/floxed-EGFP mice (P0 mice), in which NCDCs remain labeled with green fluorescent protein (GFP) even after birth. We observed proliferation and differentiation of NCDCs harvested from whisker pad in adult P0 mice.
NCDCs harvested from whisker pad proliferate stably in certain conditions, and the expression of neural crest-related genes was observed.Our findings demonstrate that NCDCs have the potential to differentiate into osteoblasts, adipocytes, and neuronal cells. Expression of odontoblast marker genes was confirmed at the contact aerials of the respective cells when co-cultured with ameloblasts and NCDCs.Hard tissue regenerative capacity of NCDCs also have been evaluated in vivo.
NCDCs from the whisker pad , which are easily accessible in adult, can be cultured and may be a useful cell source for regeneration of the hard tissue.
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| Free Research Field |
医歯薬学
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