2014 Fiscal Year Final Research Report
Analysis of physiological role of mitochondrial sphingomyelinase in the regulation of cell death
| Project/Area Number |
25870705
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental and hygienic pharmacy
Applied molecular and cellular biology
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | スフィンゴミエリナーゼ / セラミド / アポトーシス / ミトコンドリア / スフィンゴ脂質 |
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| Outline of Final Research Achievements |
Mitochondrial sphingomyelinase (mitSMase) is novel SMase that catalyzes hydrolysis of sphingomyelin to ceramide and phosphocholine. Although it was known that the ceramide generation in mitochondria was observed in various cell death processes, it was little known about the physiological role of ceramide and the generating enzymes. In this study, we investigated the possibility that mitSMase was the ceramide producing enzyme in mitochondria and the role of mitSMase in cell death. As a result, we have demonstrated that mitSMase is localized in outer membrane of mitochondria and its C terminal region that contain enzymatic domain is faced to cytoplasm. Furthermore, we have cleared that mitSMase suppresses etoposide-induced apoptosis by depleting injured mitochondria via mitophagy.
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| Free Research Field |
生化学
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