2013 Fiscal Year Final Research Report

Studies on the mechanism for goadsporin resistance

Research Project

Project/Area Number	25892008
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Applied microbiology
Research Institution	The University of Tokyo
Principal Investigator	OZAKI Taro 東京大学, 農学生命科学研究科, 特任助教 (40709060)
Project Period (FY)	2013-08-30 – 2014-03-31
Keywords	抗生物質
Research Abstract	Goadsporin is an antibiotic which inhibits the growth of actinomycetes. As the molecular target of goadsporin was proposed to be unprecedented, it was expected that the characterization of the mechanism of goadsporin action would lead to the development of drugs which possessed novel mechanism of action. This study aimed to reveal the mechanism of goadsporin action. Ffh, a possible target of goadsporin was successfully expressed as a soluble protein in Escherichia coli. GodI, a goadsporin-resistant Ffh homologue was also obtained as a recombinant protein. Using recombinant Ffh and GodI, crystallization experiments were performed. As a result, microcrystal-like objects were obtained in some conditions.