2014 Fiscal Year Final Research Report
Screening of a cofactor of DUX4, a candidate causative gene for facioscapulohumeral muscular dystrophy
| Project/Area Number |
25893046
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2013-08-30 – 2015-03-31
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| Keywords | 遺伝性筋疾患 / 筋ジストロフィー / 転写因子 / 細胞死 |
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| Outline of Final Research Achievements |
To screen the regulatory factor of DUX4, a leading candidate gene for FSHD, we tried yeast two-hybrid screening with DUX4 C-terminal region. However, the DUX4 C-terminal region fused to GAL4 DNA-binding domain showed extremely strong transcriptional activity in yeast cells, we found that it was difficult to identify DUX4 binding protein by this method. Next, we tried GST pull-down screening with GST-DUX4 C-terminus fusion protein and human muscle cell lysate. As the result, a low molecular band was detected in the elute of GST-DUX4 pull-down. We are currently trying to identify this protein.
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| Free Research Field |
分子生物学
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