Analyses of hematopoietic stem cell division patterns in biomimetic culture conditions and optimization of culture condition for hematopoietic stem cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 25893234
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: Research Institute, International Medical Center of Japan (2014); Keio University (2013)
• Principal Investigator: IKUSHIMA Yoshiko 独立行政法人国立国際医療研究センター, その他部局等, 上級研究員 (00571366)
• Co-Investigator(Renkei-kenkyūsha): ARAI Fumio 九州大学, 応用幹細胞医科学部門・幹細胞再生修復医学分野, 教授 (90365403) ; HOSOKAWA Kentaro 九州大学, 応用幹細胞医科学部門・幹細胞再生修復医学分野, 助教 (90569584)
• Research Collaborator: SUDA Toshio ; MACARTHUR Ben D. ; STUMPF Patrich S ; LUTOLF Matthias P.
• Project Period (FY): 2013-08-30 – 2015-03-31
• Keywords: 造血幹細胞 / 娘幹細胞 / 単一細胞 / 幹細胞維持 / ニッチ分子

Outline of Final Research Achievements

We investigated hematopoietic stem cell (HSC) division patterns at the single cell level based on gene expression profiles of paired daughter cells (PDCs) derived from highly purified long-term HSCs. Also we analyzed the frequency of daughter stem cells with long-term bone marrow (BM) reconstitution activity in PDCs by limiting dilution competitive BM transplantation assays. We revealed that HSCs cultured on fibronectin-coated PEG hydrogel microwells with Angpt1 produce more daughter stem cells compared to HSCs cultured on fibronectin-coated normal cell culture dish without Angpt1. Our data indicated that biomimetic cell culture condition could maintain HSCs in vitro.

Free Research Field

幹細胞医学