Myokines and their physiological roles

2016 Fiscal Year Final Research Report

• Project/Area Number: 26242068
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Sports science
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: Fujii Nobuharu L 首都大学東京, 人間健康科学研究科, 教授 (40509296)
• Co-Investigator(Kenkyū-buntansha): 眞鍋 康子 首都大学東京, 人間健康科学研究科, 准教授 (60467412) ; 中川 嘉 筑波大学, 国際統合睡眠医科学研究機構, 准教授 (80361351) ; 井上 菜穂子 日本大学, 生物資源科学部, 助教 (00509515) ; 古市 泰郎 首都大学東京, 人間健康科学研究科, 助教 (40733035)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: マイオカイン / 骨格筋 / 筋収縮 / 遺伝子組み換えマウス / 遺伝子組み換えショウジョウバエ

Outline of Final Research Achievements

The purpose of this study is to find myokines secreted by skeletal muscle cells and elucidates their physiological roles. Conditional cell culture media of C2C12 myotubes were provided to mass analysis after contraction to identify new myokines. mRNA was isolated from the C2C12 myotubes after contraction and also provided to DNA micro array analysis. As a result, we had around 40 candidates of novel myokines. Then, each candidate molecule was knocked down in skeletal muscle-specific manner in drosophila. We observed shorten life span in myokine A knocked down drosophila. On the other hand, we observed prolonged life span in myokine B knocked down drosophila. Next, we generated skeletal muscle specific myokine A over expression mice. The transgenic mice had higher glucose metabolism rate and better result of glucose tolerance test compared to wild type mice.

Free Research Field

代謝内分泌科学　運動分子生物学