Development of novel analytical NMR system for regenerative medical materials and its application for silk artificial vascular grafts

2017 Fiscal Year Final Research Report

• Project/Area Number: 26248050
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Polymer/Textile materials
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Asakura Tetsuo 東京農工大学, 工学(系)研究科(研究院), 名誉教授 (30139208)
• Co-Investigator(Kenkyū-buntansha): 田中 綾 東京農工大学, (連合)農学研究科(研究院), 准教授 (70334480) ; 佐田 政隆 徳島大学, 大学院医歯薬学研究部(医学系), 教授 (80345214) ; 山下 義裕 大阪成蹊短期大学, 生活デザイン学科, 准教授 (00275166) ; 中澤 靖元 東京農工大学, 工学(系)研究科(研究院), 准教授 (20456255)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 再生医療材料 / 固体NMR構造解析 / 絹の構造とダイナミクス / 絹と水の相互作用 / 小口径絹人工血管 / 絹スポンジコーティング / 動物移植評価実験

Outline of Final Research Achievements

In order to develop excellent biomaterials used in the water-containing state in human body, new NMR methods i.e., 13C solid state NMR, 1H solid state NMR and 2H solution relaxation NMR methods for analysis of hydrated materials were developed. These were applied to obtain the detailed information on the structure and dynamics of hydrated silk, and dynamics of water molecules interacted with silk, which was used to optimize the coating of silk on the silk bases. The artificial silk grafts were prepared and implanted in mouse, rat and dog to evaluate the ability of the graft. By repeating NMR analysis, preparation of the graft and animal implantation, the artificial silk graft with small diameter was developed.

Free Research Field

化学