Mechanism of enrichment of aromatic compound-degrading bacteria in rhizosphere of aquatic plants, and its application to remediation technology

2017 Fiscal Year Final Research Report

• Project/Area Number: 26281041
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Modeling and technologies for environmental conservation and remediation
• Research Institution: Osaka University
• Principal Investigator: Ike Michihiko 大阪大学, 工学研究科, 教授 (40222856)
• Co-Investigator(Kenkyū-buntansha): 黒田 真史 大阪大学, 工学研究科, 助教 (20511786) ; 惣田 訓 立命館大学, 理工学部, 教授 (30322176)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: バイオレメディエーション / ウキクサ / 根圏細菌 / 芳香族化合物 / Sphingobium fuliginis

Outline of Final Research Achievements

Rhizoremediation is an environmentally-sound promising technology that utilizes aquatic plants and rhizobacteria to cleanup polluted environment. This study aimed to reveal a mechanism of accelerated degradation of aromatic compounds in the rhizosphere of duckweed. Using 4-tert-butylphenol (4tBP) as a model aromatic compound, bacterial community in the rhizosphere of 4tBP-degrading duckweed was analyzed. Bacterial community of duckweed rhizosphere, where bacteria belonging to family Pseudomonadaceae and Sphingomonadaceae were dominated, was significantly different from that of environmental water. Transcriptomic analysis of 4tBP-degrading Sphingobium fuliginis OMI, originally isolated from duckweed rhizosphere, revealed that pivalate degradation pathway is related to the 4tBP degradation.

Free Research Field

生物環境工学