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2016 Fiscal Year Annual Research Report

Development of biomaterials based on analysis of molecular processes at cell-material interfaces

Research Project

Project/Area Number 26282118
Research InstitutionTokyo Institute of Technology

Principal Investigator

林 智広  東京工業大学, 物質理工学院, 准教授 (30401574)

Co-Investigator(Kenkyū-buntansha) 原 正彦  東京工業大学, 物質理工学院, 教授 (50181003)
Project Period (FY) 2014-04-01 – 2017-03-31
Keywordsバイオインターフェース
Outline of Annual Research Achievements

本研究の目的は、足場材料と細胞(接着斑)、細胞と培養液(細胞膜表面)の2つの界面における分子プロセスを、プローブ顕微鏡及び近接場光を用いた局所分光・力学測定で同時に解析する。材料―細胞界面の足場タンパク質の量・構造の時間発展(細胞の接着斑)、ナノスケール分解能で細胞膜中の脂質分子・膜タンパク質の面内分布、細胞膜の局所的力学特性・水和構造(細胞膜表面)を解析し、細胞の運命(接着、増殖、細胞死、がん化など)、細胞の薬剤への感受性を決定する要因を包括的に議論する。細胞の足場となるタンパク質の層の分析を行い、細胞応答との相関を定量的に解析を行うことであった。上記の技術は開発に成功し、以下の2つの知見を得た。
1.細胞・生体組織の初期応答には吸着タンパク質層が重要な役割を果たしており、巨視的な生体適合性にも深く関わっている。しかし、タンパク質層を形成するタンパク質の構造、組成などを包括的に解析した研究はない。我々は独自の組成分析手法を用いて、血清中から人工材料表面に吸着したタンパク質の組成を定量的に分析した。さらに、血清タンパク質の吸着後の構造変化の分析と組み合わせて、接着細胞の初期応答との相関を見出した。
2. 分子認識サイトのナノスケール分解能リアルタイムイメージングは、バイオセンサーの評価、細胞膜上の受容体タンパク質の分布の有力な解析手法となる。ここでは、Streptavidinを固定した基板とBiotinを修飾した探針を使用し、光熱励振機構を組み合わせた振幅変調型原子間力顕微鏡の位相差イメージングで、生体分子1分子レベルのナノスケール高速イメージングに成功した。

Research Progress Status

28年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

28年度が最終年度であるため、記入しない。

Causes of Carryover

28年度が最終年度であるため、記入しない。

Expenditure Plan for Carryover Budget

28年度が最終年度であるため、記入しない。

  • Research Products

    (47 results)

All 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (14 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 14 results,  Open Access: 2 results,  Acknowledgement Compliant: 3 results) Presentation (30 results) (of which Int'l Joint Research: 13 results,  Invited: 8 results) Remarks (1 results)

  • [Int'l Joint Research] ナンヤン工科大学(シンガポール)

    • Country Name
      SINGAPORE
    • Counterpart Institution
      ナンヤン工科大学
  • [Int'l Joint Research] ハンヤン大学(韓国)

    • Country Name
      KOREA (REP. OF KOREA)
    • Counterpart Institution
      ハンヤン大学
  • [Journal Article] Standing-Up Phase of Hexanedithiol Self-Assembled Monolayers on Au(111) Induced by Displacement Reaction2017

    • Author(s)
      Myoung-Soo Han Kyungbin Ku Hungu Kang Rina Ohashi Tomohiro Hayashi Masahiko Hara Jaegeun Noh
    • Journal Title

      Jounal of Nanosicence and Nanotechnology

      Volume: 17 Pages: 5780-5783

    • DOI

      10.1166/jnn.2017.14163

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Formation and Structural Changes of 4-Fluorobenzenethiol Self-Assembled Monolayers on Au(111)2017

    • Author(s)
      Myoung-Soo Han Kyungbin Ku Hungu Kang Rina Ohashi Tomohiro Hayashi Masahiko Hara Jaegeun Noh
    • Journal Title

      Jounal of Nanosicence and Nanotechnology

      Volume: 17 Pages: 5597-5600

    • DOI

      10.1166/jnn.2017.14162

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Raman imaging analysis of local crystal structures in LiCoO2 thin films calcined at different temperature2017

    • Author(s)
      K. Hara T. Yano K. Suzuki M. Hirayama T. Hayashi R. Kanno M. Hara
    • Journal Title

      Anal. Sci.

      Volume: 印刷中 Pages: 印刷中

    • Peer Reviewed
  • [Journal Article] Surface force and vibrational spectroscopic analyses of interfacial water molecules in the vicinity of methoxy-tri(ethylene glycol)-terminated monolayers: mechanisms underlying the effect of lateral packing density on bioinertness2017

    • Author(s)
      Sekine, T. Asatyas, S. Sato, C. Morita, S. Tanaka, M. Hayashi, T.
    • Journal Title

      J Biomater Sci Polym Ed

      Volume: - Pages: 1-13

    • DOI

      10.1080/09205063.2017.1303120

    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Surface Force Analysis of Pyrite (FeS2): Its Reactivity to Amino Acid Adsorption2016

    • Author(s)
      Ganbaatar, Narangerel Matsuzaki, Nina Nakazawa, Yuya Afrin, Rehana Aono, Masashi Yano, Taka-aki Hayashi, Tomohiro Hara, Masahiko
    • Journal Title

      Advances in Materials Physics and Chemistry

      Volume: 06 Pages: 167-176

    • DOI

      10.4236/ampc.2016.67018

    • Peer Reviewed / Open Access
  • [Journal Article] Formation and Structure of Self-Assembled Monolayers by Adsorption of Octaneselenocyanate on Au(111)2016

    • Author(s)
      Han, S. Kang, H. Park, J. B. Hayashi, T. Hara, M. Noh, J.
    • Journal Title

      Journal of Nanoscience and Nanotechnology

      Volume: 16 Pages: 8610-8613

    • DOI

      10.1166/jnn.2016.12509

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Comparative Study for Displacement of Cyclohexanethiolate and Cyclohaxaneselenolate Self- Assembled Monolayers on Au(111) by Octanethiols2016

    • Author(s)
      Jeong, H. Kang, H. Han, S. Park, H. Han, J. Hayashi, T. Hara, M. Noh, J.
    • Journal Title

      Journal of Nanoscience and Nanotechnology

      Volume: 16 Pages: 8508-8512

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Terminal-Specific Interaction between Double-Stranded DNA Layers: Colloidal Dispersion Behavior and Surface Force2016

    • Author(s)
      Kanayama, N. Sekine, T. Ozasa, K. Kishi, S. Nyu, T. Hayashi, T. Maeda, M.
    • Journal Title

      Langmuir

      Volume: 32 Pages: 13296-13304

    • DOI

      10.1021/acs.langmuir.6b03470

    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effect of Solution Concentration on the Formation of Ordered Domains in Pentachlorobenzenethiol Self-Assembled Monolayers on Au(111)2016

    • Author(s)
      Kang, H. Ito, E. Hayashi, T. Hara, M. Noh, J.
    • Journal Title

      J Nanosci Nanotechnol

      Volume: 16 Pages: 6360-3

    • DOI

      10.1166/jnn.2016.12115

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Correlating single-molecule and ensemble-average measurements of peptide adsorption onto different inorganic materials2016

    • Author(s)
      Kim, S. O. Jackman, J. A. Mochizuki, M. Yoon, B. K. Hayashi, T. Cho, N. J.
    • Journal Title

      Phys Chem Chem Phys

      Volume: 18 Pages: 14454-9

    • DOI

      10.1039/c6cp01168c

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Striped Phase of 3-Hexylthiophene Self-Assembled Monolayers on Au(111) from Vapor Phase Deposition2016

    • Author(s)
      Youngwoo Kim Hungu Kang Azuho Tsunoi Tomohiro Hayashi Masahiko Hara Jaegeun Noh
    • Journal Title

      Journal of Nanoscience and Nanotechnology

      Volume: 16 Pages: 2792-2795

    • DOI

      10.1166/jnn.2016.11097

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Thiol Molecules as Temperature Sensors for Surface-enhanced Raman Scattering Measurements of Heat-sensitive Materials2016

    • Author(s)
      Mochizuki, M. Asatyas, S. Suthiwanich, K. Hayashi, T.
    • Journal Title

      Chemistry Letters

      Volume: 45 Pages: 1207-1209

    • DOI

      10.1246/cl.160572

    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Fano resonant all-dielectric core/shell nanoparticles with ultrahigh scattering directionality in the visible region2016

    • Author(s)
      Tsuchimoto, Y. Yano, T. A. Hayashi, T. Hara, M.
    • Journal Title

      Opt Express

      Volume: 24 Pages: 14451-62

    • DOI

      10.1364/OE.24.014451

    • Peer Reviewed
  • [Journal Article] Laser-scanning-assisted tip-enhanced optical microscopy for robust optical nano-spectroscopy2016

    • Author(s)
      Taka-aki Yano Yuta Tsuchimoto Masahito Mochizuki Tomohiro Hayashi Masahiko Hara
    • Journal Title

      Applied Spectroscopy

      Volume: 70 Pages: 1239-1243

    • DOI

      10.1177/0003702816652369

    • Peer Reviewed
  • [Presentation] Direct measurement of blunt end stacking between DNA layers by atomic force microscopy: For understanding mechanisms underlying self-assembly process of DNA molecules2017

    • Author(s)
      Taito Sekine Naoki Kanayama Kazunari Ozasa Takashi Nyu Tomohiro Hayashi Mizuo Maeda
    • Organizer
      IUMRS-ICAM2017
    • Place of Presentation
      京都大学 吉田キャンパス
    • Year and Date
      2017-08-29
    • Int'l Joint Research
  • [Presentation] Direct measurement of blunt end stacking between DNA molecules by atomic force microscopy2017

    • Author(s)
      Taito Sekine Naoki Kanayama Kazunari Ozasa Takashi Nyu Tomohiro Hayashi Mizuo Maeda
    • Organizer
      日本化学会 第97春季年会
    • Place of Presentation
      慶應義塾大学 日吉キャンパス
    • Year and Date
      2017-03-18
  • [Presentation] 振幅変調型原子間力顕微鏡を用いた分子認識サイトの高速ナノスケールイメージング2017

    • Author(s)
      丹生隆 前川達洋 SUTHIWANICH Kasinan 林智広
    • Organizer
      第64回応用物理学会春期学術講演会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2017-03-18
  • [Presentation] 材料表面上の吸着タンパク質の組成・構造分析2017

    • Author(s)
      廣原周 前川逹洋 丹生隆 関根泰斗 林智広
    • Organizer
      第64回応用物理学会春期学術講演会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2017-03-17
  • [Presentation] プローブ顕微鏡・近接場光学を用いたバイオ界面の解析2017

    • Author(s)
      林智広
    • Organizer
      新世代光源で切り拓く物質科学と生命科学の融合領域(短期セミナー)
    • Place of Presentation
      東京大学 物性研究所
    • Year and Date
      2017-03-07
    • Invited
  • [Presentation] Effect of molecular packing density on bioinertness of self-assembled monolayers of methoxy-tri(ethylene-glycol)-terminated alkanethiols2017

    • Author(s)
      Syifa Asatyas Taito Sekine Shigeaki Morita Tomohiro Hayashi
    • Organizer
      MANA International Symposium 2017
    • Place of Presentation
      エポカルつくば
    • Year and Date
      2017-03-01
    • Int'l Joint Research
  • [Presentation] Identification of adsorbed proteins on self-assembled monolayers from serum using MALDI-ToF-MS2017

    • Author(s)
      Makoto Hirohara Tatsuhiro Maekawa Yoshiki Mizushita Takashi Nyu Taito Sekine Tomohiro Hayashi
    • Organizer
      MANA International Symposium 2017
    • Place of Presentation
      エポカルつくば
    • Year and Date
      2017-03-01
    • Int'l Joint Research
  • [Presentation] マテリアルズ・インフォマティクスによる生体材料表面設計2017

    • Author(s)
      林智広
    • Organizer
      表面科学セミナー2017 物質・材料開発の新潮流 -マテリアルズ・インフォマティクスの最前線-
    • Place of Presentation
      東京理科大学
    • Year and Date
      2017-02-10
    • Invited
  • [Presentation] On-chip proteomic analysis: investigation of scaffold proteins and correlation with cell behavior2016

    • Author(s)
      Tomohiro Hayashi
    • Organizer
      第26回日本MRS年次大会
    • Place of Presentation
      横浜文化会館
    • Year and Date
      2016-12-20
    • Invited
  • [Presentation] Correlation between Physicochemical Properties of Self-Assembled Monolayers and Protein Adsorption Analyzed with Artificial Neural Network2016

    • Author(s)
      Tomohiro Hayashi
    • Organizer
      第26回日本MRS年次大会
    • Place of Presentation
      横浜文化会館
    • Year and Date
      2016-12-19
    • Invited
  • [Presentation] Molecular recognition imaging at nanoscales2016

    • Author(s)
      Takashi Nyu Tatsuhiro Maekawa Kasinan Suthiwanich Tomohiro Hayashi
    • Organizer
      12th International Conference on Nano-Molecular Electronics (ICNME 2016)
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2016-12-14
    • Int'l Joint Research / Invited
  • [Presentation] 階層的バイオ界面における分子プロセスの解析: 細胞応答決定のメカニズムの理解から制御へ2016

    • Author(s)
      林智広
    • Organizer
      公益社団法人 日本セラミックス協会 第3回 ナノバイオセラミクスによる細胞機能制御テクノロジー研究会
    • Place of Presentation
      まちなかキャンパス長岡
    • Year and Date
      2016-12-08
    • Invited
  • [Presentation] Surface force and vibrational spectroscopic analyses about interfacial water of oligo(ethylene glycol)-terminated monolayers: To understand mechanisms underlying "bioinertness"2016

    • Author(s)
      Taito Sekine Syifa Asatyas Shigeaki Morita Masaru Tanaka Tomohiro Hayashi
    • Organizer
      3rd International Conference on Biomaterials Science (ICBS2016)
    • Place of Presentation
      University of Tokyo
    • Year and Date
      2016-11-28
    • Int'l Joint Research
  • [Presentation] 生体分子・細胞の応答を決定する界面の水分子2016

    • Author(s)
      林智広
    • Organizer
      日本表面科学会関東支部 第5回関東支部セミナー 「表面・界面の水が拓くものづくりの未来」
    • Place of Presentation
      東京大学 本郷キャンパス
    • Year and Date
      2016-11-26
    • Invited
  • [Presentation] AM-AFMを用いた高速分子認識イベントマッピング2016

    • Author(s)
      前川達洋 丹生隆 Kasinan Suthiwanich 林智広
    • Organizer
      日本表面科学会 第4回関東支部セミナー
    • Place of Presentation
      東京大学 理学部化学本館講堂
    • Year and Date
      2016-10-18
  • [Presentation] 探針増強ラマン分光法のバイオ応用に向けた探針の温度測定と温度上昇抑制技術の開発2016

    • Author(s)
      望月誠仁 Ganchimeg Lkhamsuren 矢野隆章 原正彦 林智広
    • Organizer
      日本表面科学会 第4回関東支部セミナー
    • Place of Presentation
      東京大学 理学部化学本館講堂
    • Year and Date
      2016-10-18
  • [Presentation] 原子間力顕微鏡と振動分光で見る単分子膜界面での水分子の振る舞い-生体分子吸着忌避における界面水分子の役割の解明に向けて-2016

    • Author(s)
      関根泰斗 Syifa Asatyas 森田成昭 田中賢 林智広
    • Organizer
      日本表面科学会 第4回関東支部セミナー
    • Place of Presentation
      東京大学 理学部化学本館講堂
    • Year and Date
      2016-10-18
  • [Presentation] Correlation between physicochemical properties of self-assembled monolayers and protein adsorption investigated by using an artificial neural network2016

    • Author(s)
      Rudolf Jason Kwaria Tomohiro Hayashi
    • Organizer
      Asia Nano 2016
    • Place of Presentation
      北海道 札幌市 札幌コンファレンスセンター
    • Year and Date
      2016-10-13
    • Int'l Joint Research
  • [Presentation] In-situ molecular recognition imaging at a nanoscale by atomic force microscopy2016

    • Author(s)
      Takashi Nyu Tomohiro Hayashi
    • Organizer
      Asia Nano 2016
    • Place of Presentation
      北海道 札幌市 札幌コンファレンスセンター
    • Year and Date
      2016-10-13
    • Int'l Joint Research
  • [Presentation] Interaction of prebiotic biomolecules with mineral surface using single molecule dynamic force spectroscopy2016

    • Author(s)
      Rehana Afrin Narangerel Ganbaatar Takaaki Yano Tomohiro Hayashi Masashi Aono Masahiko Hara
    • Organizer
      Asia Nano
    • Place of Presentation
      北海道札幌市 (札幌コンファレンスセンター)
    • Year and Date
      2016-10-11
    • Int'l Joint Research
  • [Presentation] Tip-enhanced THz-Raman spectroscopy: an all-optical method for precise determination and control of local temperature at the nanoscale2016

    • Author(s)
      Maria Balois Norihiko Hayazawa Francesca Celine Catalan Satoshi Kawata Takuo Tanaka Taka-aki Yano Tomohiro Hayashi
    • Organizer
      応用物理学会秋季学術講演会
    • Place of Presentation
      新潟県新潟市 朱雀メッセ
    • Year and Date
      2016-09-16
  • [Presentation] Analysis of Correlation between Physicochemical Properties of Self-Assembled Monolayers and Protein Adsorption Using Arti cial Neural Network2016

    • Author(s)
      Rudolf Jason Kwaria Tomohiro Hayashi
    • Organizer
      応用物理学会秋季学術講演会
    • Place of Presentation
      新潟県新潟市 朱雀メッセ
    • Year and Date
      2016-09-14
  • [Presentation] 振幅変調型原子間力顕微鏡を用いた分子認識イベントの高速ナノスケールマッピング2016

    • Author(s)
      丹生隆 大橋りな 林智広
    • Organizer
      応用物理学会秋季学術講演会
    • Place of Presentation
      新潟県新潟市 朱雀メッセ
    • Year and Date
      2016-09-13
  • [Presentation] 走査型プローブ顕微鏡を用いた表面・界面の制御・評価2016

    • Author(s)
      林智広
    • Organizer
      応用物理学会 有機ナノ界面制御素子(NICE)研究会
    • Place of Presentation
      新潟大学 駅南キャンパス「ときめいと」
    • Year and Date
      2016-09-12
    • Invited
  • [Presentation] Temperature Determination at the Nanoscale via Tip-enhanced THz-Raman Spectroscopy2016

    • Author(s)
      M. Balois N. Hayazawa F. Catalan S. Kawata T. Tanaka T. Yano T. Hayashi
    • Organizer
      The 14th International Conference of Near-Field Optics, Nanophotonics and Related Techniques (NFO14)
    • Place of Presentation
      アクトシティー浜松
    • Year and Date
      2016-09-05
    • Int'l Joint Research
  • [Presentation] Evaluation of affinity of material -binding peptides to materials by atomic force microscopy2016

    • Author(s)
      Masahito Mochizuki Masahiro Oguchi Seong-Oh Kim Tetsu Ogawa Ganchimeg Lkhamsuren Nam-Joon Cho Tomohiro Hayashi
    • Organizer
      KJF-ICOMEP 2016
    • Place of Presentation
      ACROS Fukuoka, Japan
    • Year and Date
      2016-09-05
    • Int'l Joint Research
  • [Presentation] Secret of bioinertness of monolayers of oligo(ethylene glycol)-terminated alkanethiols: Surface force and vibrational studies2016

    • Author(s)
      Taito Sekine Syifa Asatyas Shigeaki Morita Masaru Tanaka Tomohiro Hayashi
    • Organizer
      KJF-ICOMEP 2016
    • Place of Presentation
      ACROS Fukuoka, Japan
    • Year and Date
      2016-09-05
    • Int'l Joint Research
  • [Presentation] Controlling Fano Resonances and Scattering Directionality of High-index Dielectric Nanostructures by Thermal Oxidation2016

    • Author(s)
      T. Yano Y. Tsuchimoto T. Hayashi M. Hara
    • Organizer
      NFO-14: The 14th International Conference of Near-Field Optics, Nanophotonics and Related Techniques
    • Place of Presentation
      アクトシティー浜松
    • Year and Date
      2016-09-05
    • Int'l Joint Research
  • [Presentation] All-dielectric nanostructures for low-loss field enhanced spectroscopy and imaging2016

    • Author(s)
      Taka-aki Yano Yuta Tsuchimoto Tomohiro Hayashi Masahiko Hara
    • Organizer
      Nanoimaging and Nanospectroscopy IV
    • Place of Presentation
      San Diego, California, United States
    • Year and Date
      2016-08-28
    • Int'l Joint Research
  • [Presentation] 顕微ラマン分光イメージング法による単一細胞中のホウ素化合物の濃度分布の可視化2016

    • Author(s)
      望月誠仁 佐藤伸一 Syifa Asatyas 林智広 中村浩之
    • Organizer
      第13回日本中性子捕捉療法学会学術大会
    • Place of Presentation
      東京大学
    • Year and Date
      2016-08-06
  • [Remarks] Hayashi Lab

    • URL

      http://lab.spm.jp/

URL: 

Published: 2018-01-16  

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