2016 Fiscal Year Final Research Report
Development of New Strategy of Protein Design Based on Single Phage Sorting Technique
| Project/Area Number |
26282213
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
坂本 清志 東北大学, 多元物質科学研究所, 助教 (30335228)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | タンパク質デザイン / ファージ / 一分子蛍光観察 |
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| Outline of Final Research Achievements |
We planned to develop a new method of protein design by displaying a library of mutated fluorescent proteins on the surface of phages, and selecting phages displaying desired proteins by monitoring their emission properties. We constructed M13 phages displaying C-terminal fragment of GFP on g8p, and reconstituted GFP on the phage by adding the N-terminal fragment. We could create strongly emitting phages by displaying GFP on g8p proteins. We improved the sensitivity of the single phage detecting system. We were successful in selecting the fluorescent phages and examining the number of the collected phages by colony assay method.
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| Free Research Field |
生物物理学
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