Development and Application of Integrated Micro Cell-Sorting System Enabling High-Throughput Treatment

2016 Fiscal Year Final Research Report

• Project/Area Number: 26286032
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Nano/Microsystems
• Research Institution: Chiba University
• Principal Investigator: Seki Minoru 千葉大学, 工学(系)研究科(研究院), 教授 (80206622)
• Co-Investigator(Kenkyū-buntansha): 山田 真澄 千葉大学, 工学(系)研究科(研究院), 准教授 (30546784)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: バイオセパレーション / 細胞分離 / 分離工学 / 生物工学 / マイクロ流路 / ナノマイクロシステム / 水力学的濾過 / マイクロ流体工学

Outline of Final Research Achievements

We proposed various novel cell-separation systems such as the asymmetric lattice-shaped microchannel structure, the high-density dual-depth lattice-shaped microchannel system, and the improved hydrodynamic filtration system etc., and demonstrated their usefulness as biomedical applications by realizing direct separation and recovery of leukocytes from diluted blood, one-step non-labeled concentration of blood monocyte, simultaneous realization of leukocyte/platelet-removal and erythrocyte-concentration, etc. In addition, the high throughput cell separation exceeding 15 mL/min could be realized for diluted blood samples by adopting high-density parallel microchannel, novel separation mechanism and so on. Furthermore, we also carried out efficient trapping of circulating tumor cells by cell separation device based on cell deformability, development of "Cell Processor" by microfluidic device for multi-step carrier-medium exchange. Collaborative research with companies also started.

Free Research Field

生物化学工学