2016 Fiscal Year Final Research Report
Development about photochemical detection of nuceic acids by 19F-NMR
| Project/Area Number |
26288077
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Bio-related chemistry
|
|---|
| Research Institution | Japan Advanced Institute of Science and Technology |
|---|
Principal Investigator |
Fujimoto Kenzo 北陸先端科学技術大学院大学, 先端科学技術研究科, 教授 (90293894)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
坂本 隆 北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 助教 (80423078)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | １９FーNMR / 核酸検出 / ケミカルシフト |
|---|
| Outline of Final Research Achievements |
We demonstrated an approach for the chemical shift-based detection of nucleic acids based on CNVK (cyanovinylcarobazole) mediated DNA/RNA photo-cross-linking. The 19F NMR signal of TFT(trifluoromethyl thymidine) in a DNA duplex structure was dramatically shifted from -63.2 ppm to -71.2 ppm induced by photo-cross-linking. This larger chemical shift change induced by CNVK mediated DNA photo-cross-linking enables DNA and RNA detection without confounding effects due to the influence of neighboring bases. We have also successfully demonstrated that 10 nM miRNA can be detected in a sequence-specific manner based on miRNA amplification and chemical shift caused by the photo-cross-linking of CNVK and TFT in DNA and RNA.
|
| Free Research Field |
核酸化学
|
