2016 Fiscal Year Final Research Report
Developing the Universal iPS cell lines using genetic engineering system
| Project/Area Number |
26290066
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical genome science
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 健人 東海大学, 医学部, 准教授 (50235363)
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| Research Collaborator |
SHIINA Takashi 東海大学, 医学部, 准教授 (00317744)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | iPS細胞 / 移植片拒絶回避 / HLA遺伝子 / 遺伝子欠失 / CRISPR/Cas9 / 遺伝子発現制御 / NK細胞 |
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| Outline of Final Research Achievements |
When stocked iPSCs are utilized to allograft transplantation, immune responses to transplanted cells should be controlled. Since construction of stocked iPSCs covering all HLA allo-types are beyond the realm of possibility, various approaches to overcome the difficulty are to be explored. While we easily manipulated HLA gene locus by CRISPR/Cas9 system, it was suggested that some selection cassettes harboring PB sequences is useful to select genome-edited cells. It was raised the possibility that beta;2m-deificient iPSCs would be the target of NK cells. Therefore, instead of all HLA class I, deletion of single HLA gene locus, such as HLA-A, would be effective to apply stocked iPSCs to patients who share the HLA allo-type other than the deleted HLA-A gene locus. It is also important to establish the in vivo experimental system in which human immune cells are re-constructed and the reactions to iPSC-derived allo antigens are validated.
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| Free Research Field |
分子生物学、実験動物学、発生工学、分子遺伝学
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