Molecular mechanism of the structural formation and functional regulation of the V1 rotary motor

2016 Fiscal Year Final Research Report

• Project/Area Number: 26291009
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Chiba University
• Principal Investigator: Murata Takeshi 千葉大学, 大学院理学研究科, 教授 (80415322)
• Co-Investigator(Renkei-kenkyūsha): MIZUTANI Kenji 横浜市立大学, 生命医科学研究科, 助教 (10525570) ; YAMATO Ichiro 東京理科大学, 基礎工学部, 教授 (70111458)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: V-ATPase / 回転分子モーター / X線結晶構造解析

Outline of Final Research Achievements

V-ATPases function as ATP-dependent ion pumps, the hydrophilic V1 portion is known as rotary motor in which a central axis DF complex rotates inside a hexagonally arranged catalytic A3B3 complex using ATP hydrolysis energy. We previously succeeded in obtaining the crystal structures of the A3B3 complex which is a three-fold assembly of the identical A1B1 pair, but showed asymmetrical hexamer ring structure. In this study, we elucidated the crystal structures and biochemical properties of the A-subunit, B-subunit, A1B1 complex, and A3B3 mutant. Based on these and previous findings, we propose a molecular mechanism model of the structural formation and functional regulation of the V1 rotary motor.

Free Research Field

構造生物学