In situ Protein NMR Structure Analysis

2017 Fiscal Year Final Research Report

• Project/Area Number: 26291013
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Yokohama National University (2016-2017); Osaka University (2014-2015)
• Principal Investigator: Kojima Chojiro 横浜国立大学, 大学院工学研究院, 教授 (50333563)
• Co-Investigator(Kenkyū-buntansha): 藤原 敏道 大阪大学, たんぱく質研究所, 教授 (20242381)
• Co-Investigator(Renkei-kenkyūsha): MATSUKI Yoh 大阪大学, 蛋白質研究所, 准教授 (70551498) ; SUGIKI Toshihiko 大阪大学, 蛋白質研究所, 特任助教 (70635698) ; FURUITA Kyoko 大阪大学, 蛋白質研究所, 特任助教 (30727665)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: NMR / 蛋白質 / 立体構造 / in situ

Outline of Final Research Achievements

For in situ protein NMR structure analysis, we have developed the following NMR techniques. Amino acid selective 13C-labeling and 13C-scrambling profile analysis of protein alpha and side-chain carbons in E. coli utilized for protein NMR. Protein 19F-labeling using transglutaminase for the NMR study of intermolecular interactions. Utilization of paramagnetic relaxation enhancements for high-resolution NMR structure determination of a soluble loop-rich protein with sparse NOE distance restraints.

Free Research Field

構造生命科学