2017 Fiscal Year Final Research Report
Roles of extracellular O-GlcNAc on Notch signaling and vascular development
| Project/Area Number |
26291020
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
竹下 享典 名古屋大学, 医学部附属病院, 准教授 (70444403)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | Notch / EOGT / O-GlcNAc / 血管形成 / マウス |
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| Outline of Final Research Achievements |
Notch receptors are modified with unique post-translational modifications on epidermal growth (EGF)-like repeats at the extracellular domains. Previous studies revealed that besides O-fucose and O-glucose O-GlcNAc modification occurs on EGF repeats. However, biological function of O-GlcNAc on Notch receptors remained unknown. In this study, EOGT, a glycosyltransferase catalyzing O-GlcNAc modification of EGF domains, is inactivated in mice and phenotypical analyses were performed. Results revealed that specific EGF domains of Notch1 are modified with O-GlcNAc, which regulated DLL4-mediated Notch1 activation and thus Notch-dependent vascular development in the retina.
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| Free Research Field |
生化学
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