Elucidation of regulation mechanism of the endoplasmic reticulum membrane-bound transcription factor ATF6 and its role in the evolution of vertebrates

2016 Fiscal Year Final Research Report

• Project/Area Number: 26291040
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Kyoto University
• Principal Investigator: Mori Kazutoshi 京都大学, 理学(系)研究科(研究院), 教授 (70182194)
• Co-Investigator(Renkei-kenkyūsha): OKADA Tetsuya 京都大学, 大学院理学研究科, 助教 (70378529) ; ISHIKAWA Tokiro 京都大学, 大学院理学研究科, 助教 (70632545)
• Research Collaborator: TAKEDA Shunichi 京都大学, 大学院医学研究科, 教授 ; YAMAMOTO Takashi 広島大学, 大学院理学研究科, 教授 ; KATO Koichi 岡崎統合バイオサイエンスセンター, 教授 ; NINAGAWA Satoshi 京都大学, 大学院理学研究科, 博士研究員
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: タンパク質品質管理 / 小胞体 / 転写因子 / 分子シャペロン / 小胞体関連分解 / ゴルジ体 / エスコート / 進化

Outline of Final Research Achievements

The endoplasmic reticulum (ER) membrane-bound transcription factor ATF6 is a protein with a short half-life of 2 h and constitutively subjected to glycan-dependent ER-associated degradation, although it functions as an ER stress sensor and transducer. I analyzed mannose trimming enzymes which are important for this degradation mechanism and discovered that the trimming of M9 to M8 is carried out by EDEM2 and that the trimming of M8 to M7 is carried out by EDEM1/3 (EDEM3 plays a major role). These results have completely renewed the previous model.

Free Research Field

分子生物学