Elucidation of mechanism of biosynthesis of flavonoid, nobiletin in citrus fruit

2017 Fiscal Year Final Research Report

• Project/Area Number: 26292015
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Horticultural science
• Research Institution: Shizuoka University
• Principal Investigator: Kato Masaya 静岡大学, 農学部, 教授 (10432197)
• Research Collaborator: MA Gang ; ZHANG Lancui
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: ノビレチン / フラボノイド / カンキツ / フラボノイドO-メチルトランスフェラーゼ

Outline of Final Research Achievements

The objective of the present study is to elucidate the biosynthetic pathway of nobiletin in citrus fruit. During fruit maturation, nobiletin content in ponkan mandarin was much higher than those in Satsuma mandarin and Valencia orange. High levels of the gene expression of flavonoid hydroxylase and flavonoid o-methyl-transferase (OMT) were observed in ponkan mandarin. Functional analysis of the recombinant protein of OMT showed that OMT catalyzed the reaction
n from hydroxyl group to methoxyl group of flavonoid. Thus, these results indicated that OMT plays an important role in nobiletin biosynthesis of citrus fruit.

Free Research Field

収穫後生理学