2016 Fiscal Year Final Research Report
Identification of novel plant defense activators based on ROS signaling and membrane trafficking
| Project/Area Number |
26292032
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Plant protection science
|
|---|
| Research Institution | Tokyo University of Science |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
来須 孝光 東京工科大学, 公私立大学の部局等, 助教 (50422499)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KITAHATA Nobutaka 東京理科大学, 理工学部, 助教 (10435646)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 感染防御応答 / サリチル酸 / 細胞内膜交通系 / 植物免疫活性化剤 / 抵抗性誘導剤 / 活性酸素種 / エンドソーム / 液胞 |
|---|
| Outline of Final Research Achievements |
Based on the correlation between the temporal pattern of NADPH oxidase/Rboh-mediated production of reactive oxygen species (ROS) and downstream defense responses including hypersensitive cell death, we established a high-throughput screening to identify novel compounds that modulate plant defense responses. From a total of 11,000 compounds screened, we identified compounds that activate ROS production and hypersensitive cell death in BY-2 cells. We report the effects of these chemicals on the MAMP-induced various responses in Arabidopsis including expression of various defense-related genes in planta. We also conducted comparative transcriptomic analysis using RNA-seq to reveal the effects of the chemicals on MAMP-induced gene expression. Results suggest that some chemicals activate different defense signaling pathways distinct from the known plant defense activators that activate the salicylic acid pathway.
|
| Free Research Field |
植物分子生理学
|
