Molecular mechanisms of rRNA degradation in NPV-infected insect cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 26292173
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Insect science
• Research Institution: Nagoya University
• Principal Investigator: IKEDA Motoko 名古屋大学, 生命農学研究科, 教授 (20262892)
• Co-Investigator(Kenkyū-buntansha): 永峰 俊弘 国立研究開発法人理化学研究所, 佐甲細胞情報研究室, 専任研究員 (90237553) ; 山田 早人 名古屋大学, 生命農学研究科, 助教 (70778258)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 核多角体病ウイルス / 昆虫細胞 / 抗ウイルス応答 / リボソームRNA分解 / 全タンパク質合成停止 / 宿主域決定因子

Outline of Final Research Achievements

We previously showed that rRNA degradation occurs in Bombyx cells (BM-N cells) as an antiviral immune response during abortive infection with heterologous nucleopolyhedroviruses (NPVs). In the present study, we examined the induction mechanisms of rRNA degradation in Autographa californica NPV (AcMNPV) infected BM-N cells. We identified AcMNPV p143 (ac-p143) as an inducer of rRNA degradation. And then, we investigated the domain of Ac-P143 responsible for rRNA degradation, the cellular factors interacting with Ac-P143, and the signal transduction factors responsible for induction of rRNA degradation.

Free Research Field

昆虫ウイルス学