Study on genomic basis of morphogenesis in hemimetabolous insects using novel genetic modification techniques

2016 Fiscal Year Final Research Report

• Project/Area Number: 26292176
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Insect science
• Research Institution: The University of Tokushima
• Principal Investigator: Mito Taro 徳島大学, 生物資源学部, 准教授 (80322254)
• Co-Investigator(Kenkyū-buntansha): 友成 さゆり 徳島大学, ソシオテクノサイエンス研究部, 技術専門職員 (40448345) ; 渡辺 崇人 徳島大学, 農工商連携センター, 特任助教 (30709481)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ゲノム編集 / 昆虫 / 胚発生 / 後胚発生

Outline of Final Research Achievements

We established a technical basis for in vivo analysis of expression patterns and functional analysis of genes regulating embryogenesis in Gryllus bimaculatus, a hemimetabolous insect model system. Using CRISPR/Cas9 system, we developed a gene knock-in technique in the cricket. We applied this technique to knock-in/knockout for efficient identification of knock-out individuals by detecting marker gene expression. In addition, we succeeded in observation of gene expression patterns through developmental stages via marker gene knock-in. Data of expression and function of Hox genes was obtained, including spatial expression patterns in postembryonic stages. Furthermore, we showed availability of our knock-in method to enhancer trap analysis.

Free Research Field

発生生物学・昆虫科学