2016 Fiscal Year Final Research Report
Molecular basis fo protein-crosslinking reaction by transglutaminase focusing on aberrant regulation
| Project/Area Number |
26292192
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HASHIMOTO Hisashi 名古屋大学, 生物機能利用研究センター, 助教 (30359757)
YAMANISHI Kiyofumi 兵庫医科大学, 医学部, 教授 (10182586)
Kuroda Syunichi 大阪大学, 大学院理学研究科(産業科学研究所), 教授 (60263406)
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| Research Collaborator |
TATSUKAWA Hideki 名古屋大学, 大学院創薬科学研究科, 助教
KINOSHITA Masato 京都大学, 大学院農学研究科, 助教
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 酵素 / カルシウム / 表皮 / 線維症 |
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| Outline of Final Research Achievements |
Enzymatic cross-linking reaction of proteins is carried out by transglutaminase, which consists of enzyme family in human. By the enzymatic reaction, several essential protein modifications are accomplished such as blood coagulation and epidermis formation. The enzymatic reaction is critically regulated and contribute to homeostasis. However, in the case of aberrant expression, activation, or reaction of the transglutaminase, cellular and/or tissue damage occur that will bring several kinds of diseases in human.
In order to clarify the aberrant enzymatic reactions and their products at the level of cell, tissue, and body, we investigated the variation of the enzymatic activities and substrates (products), expression pattern of the enzymes in several tissues: liver, kidney, epidermis at disease condition. Furthermore, using medaka as the model organism, we established the gene-deficient fish of transglutaminase as diseaes model.
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| Free Research Field |
応用分子細胞生物学
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