Development of the novel technique intended to the prophylaxis and treatment of neglected tropical diseases

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293034
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Nagasaki University
• Principal Investigator: SASAKI Hitoshi 長崎大学, 病院(医学系), 教授 (00170689)
• Co-Investigator(Kenkyū-buntansha): 太田 伸生 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (10143611) ; 平山 謙二 長崎大学, 熱帯医学研究所, 教授 (60189868) ; 濱野 真二郎 長崎大学, 熱帯医学研究所, 教授 (70294915)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 遺伝子ベクター / 顧みられない熱帯病 / マラリア / 住血吸虫症 / シャーガス病

Outline of Final Research Achievements

In this study, we developed the novel DNA vaccines against the neglected tropical diseases (NTD). At first, we tried to construct the DNA vector suitable for DNA vaccine. We successfully prepared the spleen-targeted gene vector by optimization of the mix ratio of pDNA, dendrigraft poly-L-lysine (DGL), and γ-polyglutamic acid (γ-PGA) and the preparation process. We applied the vector to malaria DNA vaccines. Malaria DNA nano-vaccine showed high immune induction and prolonged survival rate of malaria-infected mice. Furthermore, we applied the vector to schistosomiasis DNA vaccine. As results, schistosomiasis DNA nano-vaccine induced dominant Th1 cellular immune response and markedly reduced the egg burden in the liver.

Free Research Field

医療薬剤学、製剤学