Proteomic profiling of exosomal proteins for the early diagnosis of Alzheimer's disease and prevention of Alzheimer's disease

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293148
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Hygiene and public health
• Research Institution: Mie University
• Principal Investigator: OIKAWA Shinji 三重大学, 医学(系)研究科(研究院), 准教授 (10277006)
• Co-Investigator(Kenkyū-buntansha): 村田 真理子 三重大学, 医学(系)研究科(研究院), 教授 (10171141) ; 山嶋 哲盛 金沢大学, 医学系, 研究員 (60135077) ; 冨本 秀和 三重大学, 医学(系)研究科(研究院), 教授 (80324648)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: アルツハイマー病 / 血液 / バイオマーカー / プロテオミクス

Outline of Final Research Achievements

Alzheimer's disease (AD) is the most common cause of dementia in elderly persons. To facilitate the early diagnosis of AD, we performed protein profiling of blood in patients with mild AD. Plasma and exosomal protein samples from mild AD patients and healthy controls were analyzed using two-dimensional differential gel electrophoresis (2D-DIGE) combined with matrix-assisted laser desorption ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF/MS) followed by peptide mass fingerprinting. Three down-regulated and two up-regulated proteins were identified. Our results suggest that altered expression levels of these proteins in blood may yield candidate biomarkers for the early diagnosis of AD.

Free Research Field

予防医学