Butyrate affect lipolysis in adipocytes co-cultured with macrophages through eicosanoid mediated pathways

2016 Fiscal Year Final Research Report

• Project/Area Number: 26350166
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits
• Research Institution: Kobe Gakuin University
• Principal Investigator: Hideo Ohira 神戸学院大学, 栄養学部, 准教授 (40351762)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: Butyrate / Adipocyte / Macrophage / Lipolysis / Prostaglandin E2 / Short chain fatty acid

Outline of Final Research Achievements

Using contact or transwell co-culture methods with differentiated 3T3-L1 and RAW264.7, we examined sodium butyrate (Bt), PGE2 and involved lipolysis mechanisms. Bt significantly increased PGE2 production compared with co-culture. In this mechanisms, Bt elevated COX2 expression in macrophages and adipocytes, cPLA2 activity in macrophages compared with co-culture. As for lipolysis, co-culture increased lipolysis, whereas butyrate and PGE2 suppressed it. The PGE receptor 3 antagonist reversed the control level in PGE2 treated cells and partially reversed the control in butyrate treated cells. It is suggested butyrate may attenuate lipolysis in adipocytes co-cultured with macrophages via PGE2 mediated and non-PGE2 mediated pathways.

Free Research Field

臨床栄養学