Evaluation of relationship between dynamic cytoskeletal rearrangement and intracelluylar mechanical field by using in situ observation of stretched cell

2016 Fiscal Year Final Research Report

• Project/Area Number: 26350503
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: The University of Tokushima
• Principal Investigator: Sato Katsuya 徳島大学, 大学院理工学研究部, 講師 (10403651)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: アクチンリモデリング / バイオメカニクス / ストレッチ刺激 / 骨芽細胞 / MEMS

Outline of Final Research Achievements

In this study, we suceeded in conducting in situ time-lapse observation of stretched cell with high spatial and temporal resolution by using originally developped cell stretching MEMS device. Deformation dynamics of actin cytoskeleton under stretch application was viualized by the GFP-tagged lifeact polypeptide.
As the result of the experiment, we found that distribution of axial strain in the actin stress fiber was not uniform, and was not in accord with extracellular substrate deformation. We also found possibillity that depolymerization of actin fibers during actin cytoskeletal rearrangement under cyclic stretch application was localized in the peripheral region of the cell.

Free Research Field

細胞バイオメカニクス