2016 Fiscal Year Final Research Report
The relationship between the morphological change and the function change of alveolar cell during mechanical stretch
| Project/Area Number |
26350505
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 肺胞上皮細胞 / 細胞骨格 / 小胞輸送 / 力学刺激 |
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| Outline of Final Research Achievements |
Alveolar cells secrete pulmonary surfactant by vesicles to prevent lung collapse. Previous studies reported that this function could decrease due to the high mechanical force, such as a ventilator. On the other hand, the force would lead the cytoskeleton reconstruction and morphological change. In this study, we investigated the relationship between the morphological change and the function change.
A549 cells were cultured on the PDMS membrane and loaded by 20% strain. When the cytoskeleton was reconstructed by the strain, the vesicles were observed in the cell more than control, suggesting that the cytoskeleton might inhibit the intracellular transport by vesicles. Additionally, we developed the MEMS devise for co-cultures of endothelial and alveolar cells. In this system, each cell was cultured on the opposite sides of the PDMS membrane, respectively. Furthermore, the membrane could be stretched, resulting that the cells were loaded by the strain of breathing motion.
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| Free Research Field |
生体工学
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