2016 Fiscal Year Final Research Report
Assessment of Whole Blood Rheology and Leukocyte Activity in ex vivo Microchannel Array Model to Contribute Cardiovascular Rehabilitation Research
Project/Area Number |
26350581
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Rehabilitation science/Welfare engineering
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Research Institution | Dokkyo Medical University |
Principal Investigator |
YASU TAKANORI 獨協医科大学, 医学部, 教授 (40265278)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Keywords | 微小循環 / 血液流動性 / リハビリテーション / 運動 / 抹消動脈疾患 / 白血球 |
Outline of Final Research Achievements |
Leucocytes play an important role in the all process of the atherosclerosis. However, clinically feasible parameters of leukocyte activation in microcirculation is limited. We developed a clinically feasible assessment method of leukocyte activity and blood rheology using a microchannel flow analyzer (MCFAN) with new designed silicon chips mimic to human microvascular network. The passage time of whole blood with heparin or EDTA-2Na+heparin through the microchannel arrays was measured using MC-FAN. Number of adhesive leukocytes (/field) was counted in offline as a gold standard of leukocyte activation. Subtraction of the passage time of blood with heparin and one with EDTA-2Na + heparin was significantly correlated with adhesive leukocyte number. The delta whole blood passage time may be a novel parameter reflecting leukocyte activity.
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Free Research Field |
人間医工学
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