The roles of exercises on motor recovery and neuronal reorganization reltated with GABA systems in the focal motor stroke rats

2016 Fiscal Year Final Research Report

• Project/Area Number: 26350594
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Rehabilitation science/Welfare engineering
• Research Institution: Tokoha University
• Principal Investigator: KUMADA Tatsuro 常葉大学, 保健医療学部, 教授 (00402339)
• Co-Investigator(Renkei-kenkyūsha): TSUTSUI Yoshihiro 常葉大学, 保健医療学部, 教授 (50073135) ; AGATA Nobuhide 常葉大学, 保健医療学部, 講師 (00549313) ; UMEMURA Kazuo 浜松医科大学, 医学部, 教授 (40232912) ; HOKAMURA Kazuya 浜松医科大学, 医学部, 教授 (90436965)
• Research Collaborator: MORISHITA Saho 常葉大学, 健康プロデュース学部, 助手 (30614010) ; YOSHIKAWA Akira 昭和大学, 医学部, 助教 (90737355)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 脳梗塞モデル動物 / 運動負荷 / 神経再構築

Outline of Final Research Achievements

Although exercise therapies in the rehabilitation can promote motor recovery after cerebral infarction, the neurologic mechanism underlying them remained largely unknown. As exercise affects neurogenesis in adult brain, we examined the role of different types of exercises on the generation and lineage of neural stem cells (especially GABAergic cells) and motor recovery using the motor cortex stroke rats. Conventional motor analysis failed to detect the functional recovery during recovery period.. However, beam-walking tests with minor modifications enables us to reveal in gait deficits and show the significant effect on exercises on promotion of motor recovery. To evaluate de novo neurogenesis, we have performed lineage analysis of newly-generating neural stem cells using BrdU labeling and immunohistochemnistry. Although BrdU(+) cells were increased in stroke rats, these are appeared not to become some subpopulation of GABAergic cells.

Free Research Field

リハビリテーション医学、神経生理学