2016 Fiscal Year Final Research Report
Development of preventive markers for cerebrovascular disease using novel oxidized modification products of proteins
| Project/Area Number |
26350907
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied health science
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ニトロトリプトファン / 酸化傷害 / 脳卒中易発性高血圧発症ラット / アポリポプロテインＥ / arginosuccinate synthase |
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| Outline of Final Research Achievements |
Oxidative stress is known to participate for development of cardiovascular diseases. We have developed a new marker for oxidative stress, which is 6-nitrotryptophan (NO2Trp). We have applied this method for stroke-prone spontaneously hypertensive rat, SHRSP. We have found NO2Trp in apolipoprotein E (ApoE), in serum from SHRSP at 18 weeks of age, just before onset of the stroke. In order to clarify the relation between tissue injury and NO2Trp in ApoE, we have checked heart, brain, liver, and kidney of the SHRSP. However, we could not observed NO2Trp in ApoE in each of the tissues. Instead, we observed four other proteins in heart, 5 other proteins in brain, 10 other proteins in liver as NO2Trp containing proteins. Among them, arginosuccinate synthase in liver is important, because this enzyme is known to control formation of nitric oxide, an important regulator of hypertension. Therefore, nitration of this enzyme may participate formation of hypertension, at least partly, in SHRSP.
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| Free Research Field |
生物化学
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