Search for the drug-responsive aminoproduct during the perinatal period and analysis of its reactive mechanism.

2017 Fiscal Year Final Research Report

• Project/Area Number: 26350957
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomolecular chemistry
• Research Institution: University of Tsukuba
• Principal Investigator: KAKO Koichiro 筑波大学, 生命環境系, 講師 (60311594)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: アミノ基 / アミノプロダクト / 妊娠高血圧 / PAHマウス / MALDI-QIT-TOF/MS / LC-MS/MS

Outline of Final Research Achievements

We identified ethanolamine (Etn) and tele-methylhistamine (tMH), as aminoproducts altering in embryonic tissue and maternal blood in response to antihypertensive drugs administered to pregnancy-associated hypertensive mouse. The high level of Etn in late-gestation embryo was responsive to the treatment with both the vasoconstriction suppressant (Hdz) and the angiotensin receptor antagonist (ARB), and ameliorated to normal level.On the other hand, the increase of maternal tMH level depended on the Hdz, not ARB. Since Hdz was shown to abrogate the enzymatic activity of monoamine oxidase B, a metabolic enzyme for tMH, as dose dependent manner in vitro, it is speculated that the inhibition of monoamine oxidase B by Hdz led to the increase of maternal tMH level.

Free Research Field

ケミカルバイオロジー