Synthesis of functional NIR-emitting water dispersible silicon nanocrystals

2016 Fiscal Year Final Research Report

• Project/Area Number: 26390024
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nanomaterials chemistry
• Research Institution: National Institute for Materials Science
• Principal Investigator: Shirahata Naoto 国立研究開発法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, 准主任研究者 (80421428)
• Research Collaborator: WINNIK Francoise ; CHANDRA Sourov ; BABA Yoshinobu ; YASUI Takao ; GHOSH Batu ; TAKEGUCHI Masaki ; MASUDA Yoshitake ; NAKAMURA Jin
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: バイオイメージング / シリコン / 量子ドット / 近赤外発光 / ナノ粒子 / In-vivo / 蛍光共鳴エネルギー移動 / 水溶性

Outline of Final Research Achievements

Functional near-IR (NIR) emitting nanoparticles (NPs) adapted for two-photon excitation fluorescence cell imaging were obtained starting from octadecyl-terminated silicon nanocrystals (ncSi-OD) of narrow photoluminescence (PL) spectra having no long emission tails, continuously tunable over the 700-1000 nm window, PL quantum yields exceeding 30%, and PL lifetimes of 300 μs or longer. These NPs, consisting of a　Pluronic F127 shell and a core made up of assembled ncSi-OD kept apart by an octadecyl (OD) layer, were　readily internalized into the cytosol, but not the nucleus, of NIH3T3 cells and were non-toxic. Asymmetrical field-flow fractionation (AF4) analysis was carried out to　determine the size of the NPs in water. HiLyte Fluor　750 amine was linked via an amide link to NPs prepared with Pluronic-F127-COOH, as a first demonstration of functional NIR-emitting water dispersible ncSi-based nanoparticles.

Free Research Field

ナノ粒子科学