2016 Fiscal Year Final Research Report
Melt-like growth of protein crystals
| Project/Area Number |
26390054
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Crystal engineering
|
|---|
| Research Institution | The University of Tokushima |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 脱塩 / 遠心濃縮 / 結晶構造解析 |
|---|
| Outline of Final Research Achievements |
Salt-free protein crystallization method with centrifugal concentration apparatus has been developed. With this method, nucleation of hen egg-white lysozyme (HEWL) crystals occurs in hyper-concentrated liquid phase obtained by liquid-liquid phase separation during centrifugal concentration processes. We could successfully observed molecular steps on the obtained crystal surface. Three-dimensional structure of lysozyme molecule obtained in this method is significantly different from that obtained by normal salting-out method.
|
| Free Research Field |
結晶成長
|
