Wide-field, depth-resolved, label-free optical microscope in biological cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26390088
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Optical engineering, Photon science
• Research Institution: Ritsumeikan University
• Principal Investigator: Wataru WATANABE 立命館大学, 理工学部, 教授 (90314377)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 光学顕微鏡 / LEDアレイ / 傾斜照明 / マルチコントラスト像

Outline of Final Research Achievements

An active illumination microscope provides simultaneous acquisition of multi-contrast images such as bright-field, dark-field, and differential phase-contrast using various illumination patterns. We experimentally demonstrate the multi-contrast images of cells. The multi-contrast images of structural modifications were obtained by changing illumination patterns with an LED array and a projector for microscopic contrast enhancement. We present a method to obtain bright-field, dark-field, and DPC images and to discriminate depth structures by controlling spatial coherence of light sources. We acquired bright field, dark field, and DPC of biological cells as well as spectroscopic images and fluorescence images.

Free Research Field

応用光学