Study on Proteome Analysis Using Three-dimensional Digital Electrophoresis

2016 Fiscal Year Final Research Report

• Project/Area Number: 26410159
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Osaka Prefecture University
• Principal Investigator: Sueyoshi Kenji 大阪府立大学, 工学(系)研究科(研究院), 助教 (70552660)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: デジタル電気泳動 / ミクロスケール電気泳動 / デジタル分子ふるい分離 / デジタル等電点分離 / デジタルアフィニティ分離 / 多次元デジタル電気泳動分画

Outline of Final Research Achievements

A digital electrophoresis device was newly developed to improve resolution and sensitivity of protein analysis in complex biogenic samples as compared to conventional western blotting assay. The developed capillary cartridges filled with hydrogels having different functional groups were easily connected each other, providing the designable digital electrophoresis device containing various separation modes. The digital molecular sieving separation, digital isoelectric fractionation, and digital affinity separation were successfully conducted by the developed devices. The combination of these modes were also confirmed, which indicated that digital western blotting could be conducted by using the proposed digital electrophoresis device.

Free Research Field

分析化学