Development of novel method to make gelatin of membrane proteins suitable to utilize as functional molecules and construction of nano-materials

2016 Fiscal Year Final Research Report

• Project/Area Number: 26410177
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Nagoya Institute of Technology
• Principal Investigator: Mizuno Toshihisa 名古屋工業大学, 工学(系)研究科(研究院), 准教授 (90345950)
• Co-Investigator(Renkei-kenkyūsha): KAWAKAMI KEISUKE 大阪市立大学, 複合先端研究機構, 特任准教授 (40619904) ; NOJI TOMOYASU 大阪市立大学, 複合先端研究機構, 特任准教授 (40452205)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: Membrane protein / Surfactant / Bioorthogonal reaction / Gelation / 3D gel

Outline of Final Research Achievements

In order to establish novel method that can immobilize membrane proteins into gel materials without protein denaturation, we studied development of novel chemically reactive-solubilization surfactants for membrane proteins and construction of 3D gels encapsulating membrane protein by using the above reactive-solubilization surfactants. The reactive-solubilization surfactants were designed from DKDKC12K; the mono-alkyne-modified DKDKC12K, Alk-DKDKC12K, and the bis-modified Bis-Alk-DKDKC12K were prepared, respectively. By an in situ cross-linking reaction of Bis-azide-PEG derivatives with Alk-DKDKC12K or Bis-Alk-DKDKC12K on binding to membrane integral domain of membrane proteins in an aqueous buffer, we succeeded in making gelation of membrane proteins without protein denaturation. By applying the above gelation process to 3D printing techniques, we also succeeded in construction of 3D-structured gels encapsulating membrane proteins.

Free Research Field

Biofunctional chemistry