Development of Intelligent Cell Separation Materials with Cell Recognition

2017 Fiscal Year Final Research Report

• Project/Area Number: 26420714
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Composite materials/Surface and interface engineering
• Research Institution: Keio University (2017); Tokyo Women's Medical University (2014-2016)
• Principal Investigator: NAGASE Kenichi 慶應義塾大学, 薬学部(芝共立), 准教授 (10439838)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 細胞分離 / 細胞接着 / 温度応答性 / 再生医療 / バイオマテリアル

Outline of Final Research Achievements

In the present study, thermoresponsive cell separation materials were developed for the application in regenerative medicine. Thermoresponsive polymer, poly(N-isopropylacrylamide), was modified on glass plates, micro structured substrates, and microfibers. Various types of cells were seeded on the prepared substrates at 37℃, and detached at 20℃. Temperature-modulated cell separation was performed using the difference in cell adhesion property on the prepared substrates. Cell separation efficiency using the prepared substrates was improved by increasing the difference in cell adhesion property among cell types.

Free Research Field

生体材料