Development of techniques for patterning of differentiating cells derived from neural stem cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26420799
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Osaka Prefecture University
• Principal Investigator: Mori Hideki 大阪府立大学, 理学(系)研究科(研究院), 准教授 (30450894)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: neural stem cell / viscoelasiticity / collagen gel / endothelial cell / UV-irradiation / cell patterning

Outline of Final Research Achievements

Collagen gels were irradiated by ultra violet (UV) light for intermolecularly-cross-linking. Young’s modulus of collagen gels increased after UV-irradiation. Collagen gels were irradiated with stripe pattern using photo-masking materials. Brain capillary endothelial cells (bEnd.3) were cultured on the UV-irradiated collagen gels. bEnd.3 cells were adhered on UV-irradiated collagen gel with stripe pattern. Next, mouse neural stem/progenitor cells formed neurosphere were seeded on UV-irradiated collagen gels with stripe patterned bEnd.3 cells. After the incubation with the differentiation medium for one week, stripe pattern consisted of bEnd.3 cells and differentiating cells form neural stem/progenitor cells were formed on UV-irradiated collagen gels.

Free Research Field

生物工学、幹細胞生物学