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2016 Fiscal Year Final Research Report

Myotonic dystrophy type 1 patient-derived iPSCs for the investigation of CTG repeat instability

Research Project

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Project/Area Number 26430053
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionKyoto University

Principal Investigator

SAKURAI Hidetoshi  京都大学, iPS細胞研究所, 准教授 (80528745)

Co-Investigator(Renkei-kenkyūsha) TAKAHASHI Masanori  大阪大学, 大学院医学系研究科, 教授 (20359847)
NAKAMORI Masayuki  大阪大学, 大学院医学系研究科, 講師 (60630233)
WATANABE Akira  京都大学, iPS細胞研究所, 助教 (60506765)
Research Collaborator UEKI Junko  
Jonouchi Tatsuya  
Project Period (FY) 2014-04-01 – 2017-03-31
Keywords病態再現 / 患者由来iPS細胞 / 筋強直性ジストロフィー / CTGリピート伸長
Outline of Final Research Achievements

Myotonic dystrophy type 1 (DM1) is caused by expanded CTG repeats in DMPK gene. The expanded CTG repeats are unstable and can increase the length of the gene with age, which worsens the symptoms. In order to investigate the repeat instability, DM1 patient-derived iPSCs were generated and differentiated into cardiomyocytes, neurons and myocytes, and we precisely analyzed the CTG repeat lengths of the cells. Our DM1-iPSCs showed a gradual lengthening of CTG repeats in all cell lines depending on the passage numbers of undifferentiated cells. However, the average CTG repeat length did not change significantly after differentiation into different somatic cell types. We also evaluated the chromatin accessibility in DM1-iPSCs using ATAC-seq. The chromatin status in DM1 cardiomyocytes was closed at the DMPK locus as well as at SIX5 and its promoter region, whereas it was open in control. These findings may help clarify the role of repeat instability in the CTG repeat expansion in DM1.

Free Research Field

筋疾患学

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Published: 2018-03-22  

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