Exosome-like synthetic liposomes containing glycosphingolipids for therapy of Alzheimer's disease

2016 Fiscal Year Final Research Report

• Project/Area Number: 26440041
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Hokkaido University
• Principal Investigator: Yuyama Kohei 北海道大学, 先端生命科学研究院, 特任准教授 (80415546)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: エクソソーム / 細胞外小胞 / アルツハイマー病 / アミロイドbeta / スフィンゴ糖脂質

Outline of Final Research Achievements

Exosome is a subtype of extracellular vesicle, released from various cells including brain neurons. Neuronal cell line (N2a)-derived exosomes can bind amyloid-beta peptide (Abeta) in GSL-dependent manner and clear it through transporting into microglia. In this study, we validated that mouse neuronal exosomes, not glial ones, had abundant GSLs and could capture Abeta. Infusion of the neuronal exosomes into APP transgenic mice decreased Abeta and amyloid depositions, similar to previously reported neuroblastoma-derived exosomes. However, synthetic liposomes containing neuronal GSLs had no potency to bind Abeta. Future challenge is to construct exosome-like liposomes referred to total lipidomics data of neuronal exosomes.

Free Research Field

神経化学