Establishment of unified theory of cellular inclusion bodies including misfolded protein

2016 Fiscal Year Final Research Report

• Project/Area Number: 26440090
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Hokkaido University
• Principal Investigator: Kitamura Akira 北海道大学, 先端生命科学研究院, 助教 (10580152)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 細胞質封入体 / 筋萎縮性側索硬化症 / FRET / 蛍光相関分光法 / FRAP / TDP-43

Outline of Final Research Achievements

Accumulation of misfolded proteins due to inappropriate stabilization results in neurodegenerative diseases including amyotrophic lateral sclerosis (ALS). In this study, we clarify structural differences of carboxyl terminal fragments of ALS-causative TDP43 (TDP35 or TDP25) by using fluorescence resonance energy transfer (FRET) or related procedure. We elucidate that nuclear localization signal sequence (NLS)-tagged TDP25 can reduce cytotoxicity through the structural change.

Free Research Field

細胞生物学・生物物理学