Osmotic stress sensing and angiotensin II in body fluids homeostasis in amphibian CNS

2016 Fiscal Year Final Research Report

• Project/Area Number: 26440164
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: University of Toyama
• Principal Investigator: UCHIYAMA Minoru 富山大学, 大学院理工学研究部(理学), 客員教授 (50095072)
• Research Collaborator: MUKUDA Takao ; HARUMI Tatsuo
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 両生類 / 体液恒常性 / 脳室周囲器官 / c-Fos / アンギオテンシン受容体

Outline of Final Research Achievements

The present study aimed to investigate forebrain areas and role of angiotensin II in body fluid homeostasis in amphibian brain. Circumventricular organs lack blood-brain barrier and probably function to detect changes of extracellular fluid. Although we cloned amphibian transient receptor potential vanilloid (TRPV) 1 and TRPV4 activated Ca++ permeability in response to osmotic stress in vitro, there was no significant change of TRPV-4 mRNA expression in dehydrated frogs using real-time quantitative PCR. C-Fos immunoreactive neurons were observed in several nuclei including the paraventricular nucleus (PVN) in forebrain of Japanese tree frog. The number of c-Fos immunoreactive cells increased in these nuclei of frogs under dehydrated condition. Angiotensin II type 1 receptor was immunohistochemically located in the medial septal nucleus (MSN). There may be physiological relationship between MSN and paraventricular nucleus according to analysis of the expression of c-Fos protein.

Free Research Field

比較内分泌学