Transcriptome analysis of flower life mutant in carnation

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450048
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Horticultural science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Tanase Koji 国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門, 花き連携調整役 (30355713)
• Co-Investigator(Renkei-kenkyūsha): Onozaki Takashi 国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門 花き遺伝育種研究領域, 花き品質育種ユニット長 (90355719)
• Research Collaborator: Inamoto Fumino 国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門・花き遺伝育種研究領域, 非常勤職員 ; Uegaki Hiroko 国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門・花き遺伝育種研究領域, 非常勤職員
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: カーネーション / 花持ち / トランスクリプト-ム

Outline of Final Research Achievements

In carnation, flower life of normal cultivars is 7days without using flower preservatives. Our research grope develops several long-life flower lines and cultivars “Miracle Rouge” and “Miracle Symphony”. To achieve comprehensive information regarding the long-life flower, we conducted a transcriptome analysis of long-life flower in these carnation lines. We obtained a total of 1253 genes which differentially expressed among normal life and long life flowers. These genes contained some ethylene production related genes as up-regulated genes. And they also contained some interesting genes which function is unknown. A further expression pattern analysis of these genes using quantitative real-time PCR revealed that the expression pattern differed among normal life and long life flowers. Further functional analysis of these genes help us to elucidate the mechanism of long flower life in carnation and may facilitate the breeding practices.

Free Research Field

園芸学