Study of behavioural control mechanism throuth the male ejaculation in the sweet potato weevil

2016 Fiscal Year Annual Research Report

• Project/Area Number: 26450067
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: 熊野 了州 帯広畜産大学, 畜産学部, 准教授 (90621053)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: イモゾウムシ / 共生細菌 / 不妊虫放飼法

Outline of Annual Research Achievements

本研究では，サツマイモの難防除害虫であるイモゾウムシの不妊虫放飼効果の向上のため，（１）実験条件下では低頻度でしか生じない本種の配偶行動の発現条件の解明，（２）DNAマーカーが開発されていない本種の精液輸送のメカニズムと定量化（３）配偶行動における共生細菌の機能解明に取組んだ．その結果，（１）大気に比べ二酸化炭素濃度が高い条件でより配偶行動が高頻度で行われ，（２）微量元素ルビジウムを混入した人工飼料を用いて飼育したオス個体と交尾したメスの体内から，オス射精物に由来する微量元素の検出が可能であることが明らかになった．一方，（３）先行研究で報告された方法により，抗生物質を利用して本種の成虫体内の共生細菌除去を試みたが，完全な除去系統の作出にまでは至らなかった．今後は本申請では行わなかった高温条件での飼育による共生細菌除去系統の確立に取り組む．

Research Products

• [Presentation] Sperm marking using the trace element rubidium to improve the monitoring of the sterile sperm usage in the sterile insect technique (2017)
  • Author(s): Norikuni Kumano, Kiyohito Teruya, Kaori Tsurui, Tetsuya Toyozato
  • Organizer: International Congress of Entomology 2017
  • Place of Presentation: Florida, USA
  • Year and Date: 2017-09-25 – 2017-09-30
  • Int'l Joint Research
• [Presentation] Sexual conflict may hinder population growth through spatial sexual segregation due to counter-harassment behavior (2017)
  • Author(s): Kaori Tsurui, Tsuyoshi Ohishi, Norikuni Kumano, Kiyohito Teruya, Tetsuya Toyosato and Kazuki Tsuji
  • Organizer: International Congress of Entomology 2017
  • Place of Presentation: Florida, USA
  • Year and Date: 2017-09-25 – 2017-09-30
  • Int'l Joint Research
• [Presentation] Quantity or quality?: Reproductive development of mass-reared West Indian sweet potato weevil, Euscepes postfasciatus, under high yield conditions for eradication using sterile insect technique (2017)
  • Author(s): Kiyohito Teruya, Tsuyoshi Ohishi, Norikuni Kumano and Kaori Tsurui
  • Organizer: International Congress of Entomology 2017
  • Place of Presentation: Florida, USA
  • Year and Date: 2017-09-25 – 2017-09-30
  • Int'l Joint Research
• [Presentation] Eradication of the sweetpotato weevil, Cylas formicarius (Coleoptera: Curculionidae), from Kume Island, Okinawa, Japan by using a combination of the sterile insect technique and the male annihilation technique (2017)
  • Author(s): Dai Haraguchi, Takashi Matsuyama, Yasutsune Sadoyama, Tsuguo Kohama, Kunio Kinjo, Norikuni Kumano, Keiko Ohno-Shiromoto and Takashi Kuriwada
  • Organizer: International Congress of Entomology 2017
  • Place of Presentation: Florida, USA
  • Year and Date: 2017-09-25 – 2017-09-30
  • Int'l Joint Research