Molecular breeding of the yeast Ogataea minuta to which genetical methods are applicable

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450111
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: YOKO-O Takehiko 国立研究開発法人産業技術総合研究所, 創薬基盤研究部門, 主任研究員 (60358306)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: Ogataea minuta / メタノール資化性酵母 / 接合 / 胞子形成 / 自律複製配列 / プラスミド / GPI アンカー

Outline of Final Research Achievements

Ogataea minuta is a methylotrophic yeast species. As the structure of glycans in O. minuta is similar to that in Saccharomyces cerevisiae, we have chosen this yeast species as a host for the production of therapeutic glycoproteins. To refine the production system, we are investigating mechanisms of mating and sporulation in O. minuta, which enable genetical analysis in this species, and developing plasmids propagated in O. minuta cells.
We determined DNA sequence around MAT locus in O. minuta, and found that two MAT loci were surrounded by a couple of inverted repeat sequences, as reported in Ogataea (Hansenula) polymorpha. We clarified that the mating type is switched by inversion of the chromosomal region between two MAT loci. To identify autonomous replicating sequence (ARS) in O. minuta, we constructed plasmid library from O. minuta genomic DNA and looked for plasmids that were retained without integrating into genomic DNA. As a result, we isolated 12 candidate sequences for ARS.

Free Research Field

遺伝学、分子生物学