2016 Fiscal Year Final Research Report
Analysis of GABA formation in mushroom and its application for cultivation of GABA enriched mushroom
Project/Area Number |
26450237
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Wood science
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Research Institution | Kindai University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
FUKUTA Yasuhisa 近畿大学, 農学部, 講師 (80609602)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Keywords | グルタミン酸脱炭酸酵素 / フォスファチジルセリン脱炭酸酵素 / GABA / シイタケ / マイタケ / エノキタケ |
Outline of Final Research Achievements |
In order to evaluate the mechanisms for gamma-aminobutyric acid (GABA) accumulation in edible mushroom, we purified and characterized the enzymes for GABA formation from Grifola frondosa, Lentinula edodes and Flammulina velutipes. The enzymes from G. frondosa, and F. velutipes enzyme used only L-glutamic acid as substrate for decarboxylation reaction so that these enzymes should belong to glutamic acid decarboxylase. However, the enzyme from L. edodes can use aspartic acid, other than glutamic acid, as substrate for the reaction. The internal amino acid sequences of this enzyme showed that the enzyme should be phosphatidylserine decarboxylase (PSD) of L. edodes. However, the reaction of PSD couldn’t be detected.
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Free Research Field |
応用生物工学
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