2016 Fiscal Year Final Research Report
Host recognition of a parasite monogenean parasite Heterobothrium okamotoi for fugu Takifugu rubripes
| Project/Area Number |
26450267
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Aquatic bioproduction science
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 寄生虫 / 宿主認識 / 粘液 / 抗体 |
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| Outline of Final Research Achievements |
Fish ectoparasites make initial contact with their hosts at body surfaces, which are covered with mucosa. Fish are the most primitive vertebrates with adaptive immunity and produce and secrete IgM into mucus. In this study, we showed that a parasite Heterobothrium okamotoi utilizes IgM to recognize their host, fugu Takifugu rubripes. Oncomiracidia of H. okamotoi shed their cilia when exposed to purified mannose-binding fractions from fugu mucus. By LC-MS/MS, proteins contained in the fraction were identified as IgM and two lectins. However, although deciliation was significantly induced by the IgM and was inhibited by mannose or a specific antibody against fugu IgM, other lectins had no effect. Immunostaining showed that fugu mannose-specific IgM binds ciliated epidermal cells of oncomiracidium. Moreover, concentrations of mannose-binding IgM in gill mucus were sufficient to induce deciliation in vitro, indicating that the parasites initially use host antibodies to colonize host gills.
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| Free Research Field |
魚類免疫
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