Accumulation mechanisms of tetrodotoxin on hybrid pufferfish and its parental species

2016 Fiscal Year Final Research Report

• Project/Area Number: 26450287
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Nagasaki University
• Principal Investigator: TAKATANI Tomohiro 長崎大学, 水産・環境科学総合研究科(水産), 教授 (90304972)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Shigenori 独立行政法人水産総合研究センター, 増養殖研究所, 主任研究員 (60463105) ; MOCHIOKA Noritaka 九州大学, 大学院農学研究院, 准教授 (40212261)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: TTX / 交雑フグ / 食品衛生

Outline of Final Research Achievements

17 natural hybrid pufferfish were collected from Enshu-nada, and their parent species were identified by analysis of those mitochondrial DNA, and those anatomical distributions of toxicity were analyzed by LC/MS analysis. Two species were contained in all specimens, and they were identified as “matora” (Takifugu porphyreus x T. rublipes), and “torama” (T. rublipes x T. porphyreus), respectively. 15 specimens were toxic, toxicity level of the ovary and liver were high, and the skin showed weakly toxic. Matora specimens indicated higher toxicity than torama specimens. In the TTX administration experiment to artificial matora hybrid puffer, these mainly accumulated TTX in the skin. In the TTX administration experiment to artificial T. rublipes and T. porphyreus as a parental species, the TTX accumulation rate of both species rose by their growth. It is supposed that the toxin accumulation mechanisms are hereditary from female parental species

Free Research Field

水圏生命科学