Studies on molecular mechanisms in the acquisition of maturational competence of red seabream, Pagrus major

2017 Fiscal Year Final Research Report

• Project/Area Number: 26450298
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Fisheries Research and Education Agency
• Principal Investigator: Okuzawa Koichi 国立研究開発法人水産研究・教育機構, 増養殖研究所, センター長 (00211813)
• Co-Investigator(Kenkyū-buntansha): 宇治 督 国立研究開発法人水産研究・教育機構, 増養殖研究所, 主任研究員 (40372049) ; 風藤 行紀 国立研究開発法人水産研究・教育機構, 増養殖研究所, グループ長 (60399996) ; 藤原 篤志 国立研究開発法人水産研究・教育機構, 中央水産研究所, 主幹研究員 (30443352)
• Research Collaborator: SATOH Mana ; NYUJI Mitsuo ; YAMAGUCHI Toshiya
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 卵成熟 / 卵成熟能 / マダイ / トランスクリプトーム / 卵濾胞

Outline of Final Research Achievements

Transcriptomes were compared between maturationally competent and incompetent ovarian follicles of red seabream, Pagrus major. Gene expressions of luteinizing hormone receptor and progesterone receptors of competent follicles were 8 and 6 times higher than incompetent ones, respectively. An artificial induction of the competency by human chorionic gonadotropin (hCG) resulted in up-regulations of the genes encoding enzymes catalyzing sex steroid hormone syntheses, tight junction protein, etc. Among them, a putative gene with enhanced gene expression of around 900-fold by hCG is estimated as the key enzyme catalyzing production of maturation-inducing steroid.

Free Research Field

魚類繁殖生理学