2016 Fiscal Year Final Research Report
Study on development and application of histochemically stainable probe using fluorescent pigment dye
| Project/Area Number |
26460166
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Drug development chemistry
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| Research Institution | Hiroshima International University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | シアリダーゼ / 蛍光イメージング / シアル酸 / 組織染色 / インフルエンザ |
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| Outline of Final Research Achievements |
In this study, we developed the novel artificial sialidase substrate with 2-benzothiazol-2-yl-phenol skeltone, which is the fluorescent pigment dye.
It was revealed that the influenza infected cells had been histochemically stained with the developed sialidase substrate, and the substrate could detect the infection of not only the human influrenza virus but also the avian influenza virus, non-traditonal type (H1N1) or drug-resistant viruses. And it was easily possible to detecte the drug-resistant virusese by additon of the novel sialidase substrate and anti-influenza-neuraminidase drug in a few minute. The drug resistance property was indicated by a fluorescent focus formed by an activity of the neuraminidase, so the unknown mutation resulting the new drug resistant viruse would be detected.
The developed novel substrate is now commercially available under the support of WAKO JUNYAKU KOGYO.
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| Free Research Field |
ケミカルバイオロジー
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